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Image Search Results
Journal: Nucleic Acids Research
Article Title: RNAe: an effective method for targeted protein translation enhancement by artificial non-coding RNA with SINEB2 repeat
doi: 10.1093/nar/gkv125
Figure Lengend Snippet: Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding m7G(5′)ppp(5′)G during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).
Article Snippet: RNA with 5' cap was transcribed also by
Techniques: High Throughput Screening Assay, Transfection, Construct, CCK-8 Assay, Plasmid Preparation, In Vitro, Generated, Two Tailed Test