ribo m7g cap analog kit Search Results


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New England Biolabs m7g capping kit
M7g Capping Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hiscribe T7 Arca, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega ribo m7g cap analog kit
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New England Biolabs arca kit
Arca Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs hiscribe t7 high yield rna synthesis kit with m7g 5 ppp 5 g
Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding <t>m7G(5′)ppp(5′)G</t> during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).
Hiscribe T7 High Yield Rna Synthesis Kit With M7g 5 Ppp 5 G, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TriLink ribo m7g cap analog
Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding <t>m7G(5′)ppp(5′)G</t> during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).
Ribo M7g Cap Analog, supplied by TriLink, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs m7g ppp
Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding <t>m7G(5′)ppp(5′)G</t> during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).
M7g Ppp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Arraystar inc human m7g small rna modification microarray
Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding <t>m7G(5′)ppp(5′)G</t> during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).
Human M7g Small Rna Modification Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Arraystar inc microarray
Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding <t>m7G(5′)ppp(5′)G</t> during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).
Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech m7g rna methylation rip kit
Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding <t>m7G(5′)ppp(5′)G</t> during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).
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Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding m7G(5′)ppp(5′)G during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).

Journal: Nucleic Acids Research

Article Title: RNAe: an effective method for targeted protein translation enhancement by artificial non-coding RNA with SINEB2 repeat

doi: 10.1093/nar/gkv125

Figure Lengend Snippet: Extensive study of RNAe in high-throughput screening. HEK293T cells were transfected with RNAe and RNAi constructs corresponding to the indicated genes and CCK8 assay was conducted to detect their influence on cell proliferation. Results are shown according to the relative effects. ( A ) Comparison of RNAe and RNAi effect on genes with proliferative function. RNAe and RNAi both displayed significant effects. ( B and C ) Comparison of RNAe and RNAi effect on genes with anti-proliferative function under serum starvation (B) or normal conditions (C). In the group of anti-proliferative genes, RNAe had a significant effect under normal culture conditions (C), and RNAi under serum starvation (B). ( D and F ) RNAe constructs corresponding to the indicated genes were introduced into HEK293T calls either in the form of plasmid DNA (‘pRNAe’ group) or in vitro generated RNA (‘RNAe transcribed in vitro ’ group, generated by in vitro transcription without 5' cap) under normal conditions (D) or under serum starvation (F), respectively. RNAe showed a significant effect when delivered in either form. ( E and G ) RNAe constructs transcribed in vitro with or without 5' cap (by adding m7G(5′)ppp(5′)G during in vitro transcription procedure) were introduced into cells under normal conditions (D) or serum starvation (F), respectively, and both methods of delivery had a similar effect (mean ± SD, n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001, two tailed t -test).

Article Snippet: RNA with 5' cap was transcribed also by HiScribe™ T7 High Yield RNA Synthesis Kit with m7G(5′)ppp(5′)G (New England Biolabs, UK) added.

Techniques: High Throughput Screening Assay, Transfection, Construct, CCK-8 Assay, Plasmid Preparation, In Vitro, Generated, Two Tailed Test